Systematic deletions in the cellobiohydrolase (CBH) Cel7A from the fungus Trichoderma reesei reveal flexible loops critical for CBH activity
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چکیده
منابع مشابه
Multi-Mode Binding of Cellobiohydrolase Cel7A from Trichoderma reesei to Cellulose
Enzymatic hydrolysis of recalcitrant polysaccharides like cellulose takes place on the solid-liquid interface. Therefore the adsorption of enzymes to the solid surface is a pre-requisite for catalysis. Here we used enzymatic activity measurements with fluorescent model-substrate 4-methyl-umbelliferyl-β-D-lactoside for sensitive monitoring of the binding of cellobiohydrolase TrCel7A from Trichod...
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The mathematical formalisms for classical and quantum physics look nothing alike. This is surely one reason why physics students find it difficult to learn quantum mechanics. It is surely also one of the reasons why QM fascinates philosophers — because its formalism seems to resist comparison with the formalism of classical physics. Our goal: We want to overcome this situation of apparent Kuhni...
متن کاملProcessive action of cellobiohydrolase Cel7A from Trichoderma reesei is revealed as 'burst' kinetics on fluorescent polymeric model substrates.
Reaction conditions for the reducing-end-specific derivatization of cellulose substrates with the fluorogenic compound, anthranilic acid, have been established. Hydrolysis of fluorescence-labelled celluloses by cellobiohydrolase Cel7A from Trichoderma reesei was consistent with the active-site titration kinetics (burst kinetics), which allowed the quantification of the processivity of the enzym...
متن کاملNucleotide sequence of the cellobiohydrolase gene from Trichoderma viride.
The filamentous fungus, Trichoderma viride, secretes large amounts of several cellulolytic enzymes (1). We report here the nucleotide sequence of the gene coding for exo-cellobiohydrolase (CBH), which accounts for more than 65% of the total cellulase content. This gene was isolated from a T. viride genomic library using a 30mer oligonucleotide based on the sequence of the reported CBHI gene of ...
متن کاملMixtures of thermostable enzymes show high performance in biomass saccharification.
Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments. Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ra...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2019
ISSN: 0021-9258
DOI: 10.1074/jbc.ra118.006699